Molecular profiling of castor allergic protein

Castor seed is a potent source of allergic proteins and 2s albumin is a principal protein among them.Scientists have monitored the  expression of this protein over different developmental phases of the castor seed and plant.They were able to observe the expression of this protein  after 2 weeks of pollination and continued to exist till the completion of seed germination.Post germination events witnessed a loss in the levels of this allergen to a significant event.The researchers also detected that the protein adopts a post translational modification and found to be localized in endosperm and cotyledons of the seed during the course of its existence. Immuno-electroblotting and Quantitative PCR were the techniques applied in this research study to address the problems.

2S albumins are potent allergens from castor beans which affects the cultivation of castor in several countries as field workers find it difficult to physically handle the oil seed.Understanding the expression pattern of the allergen would assist the scientists to regulate the synthesis of this protein and ultimately generate a seed with levels of 2s albumin safe to cultivate and harvest castor.

For further reference:http://pubs.acs.org/doi/abs/10.1021/jf071272p

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