Analysis of sub-cellular components in castor seed

Castor seed is an evolved propagative material with a great molecular complexity in endosperm. Biochemical analysis of protein bodies in castor seed endosperm has been made.Protein bodies account for a major fraction in the total protein content in castor seed and is thought to involve in an important role in the mobilization of nutrients during seed development and germination.Scientists from an University in USA have isolated them from endosperm tissues and identified two principal units namely globoids and crystalloids embedded in an amorphous matrix.A protein fingerprint of buffer and detergent soluble matrix and crystalloid fractions from protein bodies was examined and revealed complexes and modified proteins including the toxin Ricin present in the soluble fraction.The principal techniques applied in this experimental study were gel electrophoresis and chromatography for resolution and analytical purposes.

Studies of such type are noteworthy to identify novel members of proteins along with their sub-cellular localization and biological functions.

For further reading: http://www.plantphysiol.org/content/58/6/710.short

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