Ricin only lethal in combination with sugar

The plant toxin ricin is one of the most poisonous naturally occurring proteins, making it an extremely dangerous bioweapon. Scientists have been searching for an effective ricin antidote for decades. However, cytotoxins like ricin also provide important insights into the molecular characteristics of cells, such as the contact points the poison uses to enter cells. Castor-Oil-Seeds2

IMBA researchers have now discovered that sugar is a key factor. The researchers identified two genes that make ricin so lethal. Fut9 and Slc35c1 regulate metabolism of a particular sugar in cells, an essential monosaccharide called fucose.


Castable Polymers made from Castor Beans could Replace Conventional Plastics

Super-tough materials derived from inedible plants are lightweight, renewable and strong enough to use in vehicles

A new polymer blend made from castor beans is stronger and lighter than conventional structural materials, and researchers say it could improve the safety and fuel efficiency of cars.Castor-Oil-Seeds2

Castor beans are notorious for containing the poison ricin, but it’s possible to turn them into polyamide 11, a thermoplastic from the nylon family of polymers. Now, scientists from Japan have developed a super-strong polymer blend by mixing polyamide 11 with the common household plastic polypropylene, that could replace many of the plastics we use today.

Poison from Castor Bean Seeds Won’t Affect Future Crops

It is well known that castor seeds contain a poisonous toxin ricin. For farmers and other agriculturists involved in cultivation of castor and other vegetable crops, there would be a feeling of uncertainty as to whether cultivation of castor and other vegetable crops can be done in the same land, due to the poisonous nature of castor seed.castoroil-seed

It should be noted that not the entire castor plant is poisonous; the toxin ricin is present only in castor seeds. Even the castor oil extracted from the seeds does not contain any toxin. Only the castor meal contains ricin. Once the plant is harvested and the residue cleaned up at the end of the growing season, there is no poison left out in the cultivated space; hence there is no harm in cultivating other vegetable crops in the same space where castor was cultivated.

Castor oil and meal has many benefits. Castor oil has been used as a purgative since long and still finds many advanced applications. Castor meal is now being utilized in some commercial repellent sprays for squirrels, rabbits, moles and other garden pests.


Genetic Engineering Methods to Overcome Toxicity Challenges in Castor Industry

The toxicity of castor, in the form of ricin, is one of the key challenges that is slowing the adoption of castor as a crop in some countries, especially in the developed parts.

Toxicity challenges in castor industry can be overcome through genetic engineering.

An example of this is the Genetic Engineering of Lesquerella fendleri for castor oil production.

  • Seed oil of Lesquerella fendleri (Lf) contains a valuable hydroxy fatty acid (HFA), lesquerolic acid (20:1OH).
  • Lf does not have biological toxic compounds, thus its oil represents an alternative source of HFA. Lesquerolic acid is derived by a 2 carbon elongation of 18:1OH.
  • By suppressing the elongation step through genetic engineering, it is possible to generate a new Lf crop that could produce 18:1OH.

Novo Synthetix, USA has followed the genetic engineering technique in castor such that it no longer produces ricin. No new DNA is introduced, mainly tweaking of genes.

Anti-cancer activity from new-world ricins

Biological systems have a complex chemistry which tend to exhibit functions under conditions of demand and proteins are one such prime classes of bio-molecules, persisting in organisms with a tremendous degree of diversity in their constituent composition and functions.Characterization of distinct candidates of proteins for functional vitality is a consensus process, independent of organism and has been followed for Castor here in the context of functional evaluation of strange variants of toxin,ricin and agglutinin in bio-medicine.

Ricin protein is complex protein with well defined lethal functional activity in human cells but its variation inside the castor plant and populations has not been elucidated extensively either on materialistic or functional basis which triggered researchers from Taiwan to initiate freak identification of ricin protein and related toxins from castor seed,richest source of castor toxins.To address this question,they adopted a protein fractionation chromatographic and electrophoresis techniques to resolve  multiple variants of ricin and evaluating the activity of purified, anomalous forms.The results indicated three principal isoforms of ricin(A,B,C) and an agglutinin, in which toxic activity was seen to a maximum level from ricin A upon oral feeding of it in mice and further observation from them revealed that the concerned protein isolate turned out to a novel member.The scientists subsequently subjected it to display its impact against tumour cells and noticed cell division inhibitory activity which was also selective and differential in mode of action to diseased cells.

It is an exciting study to see as the protein chemists followed an elegant and lucid experimental design but ended up with a potent toxin coupled to anti-tumor activity.However the thing that is to be monitored is the contrasting functional behaviour of this new toxin between an intact organism and a cancer cell line which obviously demands for a proper bio-safety evaluation to scale up the promotion of this toxin (declared as a lectin as per authour’s interpretations) for any disease management actions in humans.

For reference: http://www.sciencedirect.com/science/article/pii/0041010186901005

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Return of Castor in North America

Castor, an oil seed crop is currently confined to oriental countries to a vast extent in terms of cultivation and oil production but the demand is constantly mounting from the western countries so as to produce precious chemical derivatives from it.It is a crop relatively suitable for cultivation in West as well but  has been strongly ignored on account of its toxic properties including allergens and toxins.Research efforts have been continuously made to overcome the defined, negatives of castor and yielded appreciable results in recent years particularly in USA.

Such committed actions in castor biology are not restricted to research levels but tend to proceed further when a collaborative agreement has been made between a Canadian bio-products company Lubbock-Castagra and Texas AgriLife Research to produce castor seed with less toxin and high, sustainable yield. Reports from concerned authourities said that  encouraging the crop improvement in castor would restore the abandoned castor cultivation with desired modifications in cropping patterns of the Texas state and directly reduce the burdens of import of this plant oil commodity from countries including India.CEO of Canadian Castagra-Lubbock, Mr:Peter Roosen further added that the oil extracted from this quantitatively toxin compromised, castor would be exploited for the production of renewable bioplastics and hence carrying a tremendous potential for the generation of employment in green technology sector.Presently,the castor crop with reduced levels of toxin is at the trial stage in Texas and evaluation studies are being undertaken for a bulk cultivation, expected in 2012.

It is inspiring to watch such an activity adopted by the legal bodies in developed nations to complement the defects what they are experiencing in their environment with respect to castor. Similar sort of co-ordinated plans are mandatory to maximize the revenues from castor in India.This is well punched with reference to the promotion of end derivatives of castor oil in which India is placed way behind the Westerners in the production tally.It not alone ensures economic exchanges of profits but depicts India an industrially competitive nation at the global level.

For reference: http://media-newswire.com/release_1152924.html

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Functional significance for castor allergens

Castor seed has abundant level of proteins which are evolved spontaneously to serve the survival demands of the organism in response to the selection pressures. Among multiplicity in proteins, scientists have started focussing on three candidate proteins 2S namely albumins,Ric c1 and Ric c3 which have been earlier characterized as allergens for their biological significance.In general, bio-molecular functional definition can be arrived from analysis of its constituents and its spatial configuration that stood as bases for the functional implications in chosen castor proteins.The allergen proteins under study are found to contain a domain for digestive enzyme inhibition which made researchers to design an insect feeding bio-assay in insects Callosobruchus maculatus, Zabrotes subfasciatus, and Tenebrio molitor over their most optimal diet with parameters of insect larvae mortality and body mass gain from diet containing castor protein allergens .The results confirmed that a loss of body weight by one-fifth fraction compared to control lines which enabled researchers to arrive at the enzyme inhibitory activity from concerned plant proteins due to test domain in them.Further scientists claimed that the castor proteins are potential targets in the design of inhibitors with importance in crop protection and biomedical field.

The results seem to be promising for the feeding deterrent activity from castor proteins  but it would be convincing if an in vitro enzyme assay with gut enzymes against castor allergens and histopathological analysis of insect tissues for molecular complexes would have been performed for confirmed functional implication.

For further info: http://pubs.acs.org/doi/abs/10.1021/jf104638b

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Profiling of castor toxin by genetic amplification

Ricin toxin is a threatening agent with significance in global warfare. Scientists have been working in the development of several distinct analytical methods for the detection of toxin with improved sensitivity and specificity  but they still tend to suffer from critical limitations especially under circumstances when direct immuno probe based protein toxin profiling was made in complex biochemical samples including food stuffs that resulted in ambiguities.It stood as one of the fundamental reasons which has diverted researchers to concentrate on genetic profiling of toxin in samples for precise interpretation.

US scientists have now developed a technique called real time PCR which targets the genetic material in samples.They applied a genetic probe specific for the toxin in DNA samples isolated from milk and egg for generating a fingerprint.Experimental results showed that their probe functioned relatively well and was able to detect toxin at concentrations of 5ng per ml of milk or liquid egg.It was also reported that negative interferences from the samples were obvious but researchers were quite content with the level of detection of sub-lethal doses of toxin in biological fluids.

It is interesting to watch technique such as this since the analysts were targetting the core biomolecule,DNA which permitted them to reduce the chemical complexity of samples by fractionation for convenient profiling. Nevertheless it is vital to note that advanced PCR systems are expensive and prone for producing artifacts, also demanding for skills in the preparation of quality samples and optimization of probes in the detection of multiple variants of ricin DNA from the genomic pools of biological sources standing at different loci.

For reference: http://pubs.acs.org/doi/abs/10.1021/jf0707738

Biosensor development for ricin toxin

Ricin detection in  biological samples is of great significance as the toxin is reported to be a candidate substance for intoxication in bio-terrorism, suicide etc.By classical methods people have exploited antibody based immuno probes for detection of test toxin in materials under observation.However it suffers from certain drawbacks including sensitivity,cross-reactivity,time and technical preparation.It prompted researchers around the world to surf for alternate probes in bio-sensor developments which can effectively circumvent these problems.

Scientists from Japan have made use of natural molecular interaction behaviour of toxin and designed a novel probe to profile the toxin.By applying knowledge in ligand-receptor chemistry of ricin in biological systems,they have designed a synthetic analogue that acts as a ligand to toxin.It was immobilized onto a solid matrix to serve in stationary phase and ricin incubation was performed.The toxin bound to the test probe was assessed by a special spectroscopic technique Surface Plasmon Resonance(SPR) which indicated ricin at concentrations of 5 pg/ml in 5 minutes which the authours claimed as a benchmark in biosensor designing.The results obtained from this new probe were further validated by alternate methods for specificity.

The experimental research work was exciting as a analysts have completed a broad study and generated promising results with cutting edge technologies in the qualitative and quantitative fingerprinting of castor toxin.A technique that is pointed in nature proportionately requires professional perfection for absolute analysis.

For reference:http://www.sciencedirect.com/science/article/pii/S0956566308004016

Candidate catalyst for ricin toxin activation

Ricin toxin from castor is a dimeric toxin and initially synthesized as a toxoid but found to be processed in castor beans during the course of seed development.As the protein is localized in the cellular sub components, protein bodies of seed endosperm, researchers from UK have made their experimental investigation on concerned cell organelles of storage function for the detection of molecular factor with catalytic potential to transform the inactive toxin to its lethally active phase.Protein profiling was adopted after isolation and fractionation of crude mixtures from protein bodies to identify the specific target and the results confirmed the materialistic presence of an acid endopeptidase enzyme.The functional relevance for this enzyme over toxin transformation was evaluated by in vitro assays where active toxin release was noticed  from its precursors.

Scientists from this study were able to detect a key candidate acting as an accelerator of ricin toxicity and offered us a solution to target the molecular components in castor with flexibility for producing non toxic seeds instead of a restricted direct focus over the toxin.However it is vital to understand the specificity in this toxin-catalyst association for precise targeting to improve the application potential of castor crop.

For reference: http://www.sciencedirect.com/science/article/pii/0168945285901116

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